The use of a new cetrimide product in a selective medium for Pseudomonas pyocyanea.

نویسندگان

  • E J LOWBURY
  • A G COLLINS
چکیده

A selective medium containing 0.1 % cetrimide in nutrient agar has been used here during the past four years for the routine identification of Ps. pyocyanea in burns (Lowbury, 1951). This medium, a modification of those previously described by Harper and Cawston (1945) and by Hood (1948), has proved convenient and efficient. In October, 1953, the manufacturers announced the production of a modified "cetavlon" which was claimed to be more active against Ps. pyocyanea than the old product, and little less active against that organism than against Proteus vulgaris. In view of these observations we have made the following tests to find whether the new cetrimide is suitable for use in a selective medium for Ps. pyocyanea, and if so at what concentration it should be included.

برای دانلود متن کامل این مقاله و بیش از 32 میلیون مقاله دیگر ابتدا ثبت نام کنید

ثبت نام

اگر عضو سایت هستید لطفا وارد حساب کاربری خود شوید

منابع مشابه

Improved Culture Methods for the Detection of Ps. pyocyanea.

Williams, Clayton-Cooper, Faulkner, and Thomas (1944) drew attention to the fact that Pseudomonas pyocyanea may survive and even multiply in solutions of cetrimide (cetyl-trimethyl-ammonium-bromide). The use of this compound in selective media for the identification of Ps. pyocyanea was suggested by Harper and Cawston (1945), who recommended a concentration of 1 % in nutrient agar. This medium,...

متن کامل

Contamination of cetrimide and other fluids with Pseudomonas pyocyanea.

cream applied locally or used for holding sterile instruments might be conveying the infective agent. Soap solution, cetrimide, " dettol" and calamine lotion are commonly dispensed in large corked or stoppered bottles which are sometimes not replaced for many days, and during that time they are frequently opened. Contamination of such bottles is likely, and the survival or multiplication of som...

متن کامل

New selective medium for Pseudomonas aeruginosa with phenanthroline and 9-chloro-9-[4-(diethylamino)phenyl]-9,10-dihydro-10- phenylacridine hydrochloride (C-390).

A new selective medium (PC agar) for the isolation of Pseudomonas aeruginosa was developed, consisting of 30 micrograms of 9-chloro-9-[4-(diethylamino)phenyl]-9,10-dihydro-10-phenylacridine hydrochloride (C-390) per ml and 30 micrograms of phenanthroline per ml in Columbia agar. PC agar was superior to phenanthroline, C-390, acetamide, and cetrimide agars for the selective growth of P. aeruginosa.

متن کامل

A cabinet for the detection of fluorescent bacterial cultures.

A cabinet for detecting the fluorescence of cultures on agar media is described. The use of fluorescence as a criterion for detection of Ps. pyocyanea is compared with that of the oxidase reaction. From a series of patients with burns, all colonies of Gram-negative bacilli which gave a positive oxidase reaction yielded fluorescent subcultures on 0.03% cetrimide agar; all fluorescent cultures on...

متن کامل

Use of an improved cetrimide agar medium and other culture methods for Pseudomonas aeruginosa.

In a comparison of two selective media for Ps. aeruginosa containing 0.03% cetrimide, stronger fluorescence was obtained from growth on the medium prepared with King's medium B as the base (CTA 2); although Ps. aeruginosa was not isolated more frequently from burns on this medium than from cetrimide agar made with a Lemco base (CTA 1), results were easier to assess and there were fewer cases of...

متن کامل

ذخیره در منابع من


  با ذخیره ی این منبع در منابع من، دسترسی به آن را برای استفاده های بعدی آسان تر کنید

برای دانلود متن کامل این مقاله و بیش از 32 میلیون مقاله دیگر ابتدا ثبت نام کنید

ثبت نام

اگر عضو سایت هستید لطفا وارد حساب کاربری خود شوید

عنوان ژورنال:
  • Journal of clinical pathology

دوره 8 1  شماره 

صفحات  -

تاریخ انتشار 1955